Fully automated enzyme immunoassay system for the determination of activator-specific histamine release from basophils in whole blood.
نویسندگان
چکیده
assays as well as the fertility and oncology assays. Pooled estimates of within-run imprecision for the replicate results were calculated in each of the segments. The results are summarized in Fig. 1F. The precision for the ACS:Centaur system was demonstrated to be equivalent to that of the ACS:180 system. Diagnostic concordance between the systems was also verified using patients with results within the reference interval. Diagnostic concordance of the ACS:180 thyroid, fertility, and oncology assays on the ACS:Centaur system was .96% for all assays. Data from regression analysis and diagnostic concordance studies are summarized in Table 1.
منابع مشابه
Effect of a serum factor on IgE-mediated histamine release from whole blood.
IgE-mediated histamine release from whole blood was analyzed in 44 patients with bronchial asthma by observing maximum present release and dose-response curves of histamine release induced by anti-IgE and house dust extract. The maximum histamine release from whole blood induced by anti-IgE correlated with total serum IgE levels. There was a close correlation between allergen-induced release fr...
متن کاملThe effects of dasatinib on IgE receptor-dependent activation and histamine release in human basophils.
Dasatinib is a multitargeted drug that blocks several tyrosine kinases. Apart from its well-known antileukemic activity, the drug has attracted attention because of potential immunosuppressive and anti-inflammatory effects. We report that dasatinib at 1 microM completely blocks anti-IgE-induced histamine release in blood basophils in healthy donors, and allergen-induced release of histamine in ...
متن کاملA bispecific antibody against human IgE and human FcgammaRII that inhibits antigen-induced histamine release by human mast cells and basophils.
BACKGROUND FcgammaRIIB are low-affinity immunoglobulin (Ig)G receptors that we previously demonstrated to negatively regulate IgE-induced mast cell activation when coaggregated with FcepsilonRI. Here, we engineered and characterized a bispecific reagent capable of coaggregating FcgammaRIIB with FcepsilonRI on human mast cells and basophils. METHODS A bispecific antibody was constructed by che...
متن کاملDetection of virus-specific intrathecally synthesised immunoglobulin G with a fully automated enzyme immunoassay system
BACKGROUND The determination of virus-specific immunoglobulin G (IgG) antibodies in cerebrospinal fluid (CSF) is useful for the diagnosis of virus associated diseases of the central nervous system (CNS) and for the detection of a polyspecific intrathecal immune response in patients with multiple sclerosis. Quantification of virus-specific IgG in the CSF is frequently performed by calculation of...
متن کاملThe identification and characterization of umbilical cord blood-derived human basophils.
Cross-linking allergen-specific immunoglobin E on human peripheral blood basophils results in the release of histamine and other inflammatory mediators that initiate allergy and asthma. The signaling pathways leading from IgE binding to mediator release have not been well established, mainly due to the difficulty in obtaining adequate numbers of highly purified basophils. It was the goal of thi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Clinical chemistry
دوره 44 9 شماره
صفحات -
تاریخ انتشار 1998